关注公众号
收藏产品 产品详情
分子别名（Synonym）
HLA-A*0201 | B2M | MAGE-A4
表达区间及表达系统（Source）
Biotinylated Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Complex Protein (HLM-H82E5) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Ile 308 (HLA-A*02:01) & Ile 21 - Met 119 (B2M) & GVYDGREHTV peptide (Accession # AAA59606.1 (HLA-A*02:01) & P61769-1 (B2M) & GVYDGREHTV).
Predicted N-terminus: Gly 25 & Ile 21
Request for sequence蛋白结构（Molecular Characterization）
Biotinylated Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Complex Protein is produced by co-expression of HLA and B2M loaded with MAGE-A4 peptide.
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein migrates as 42-43 kDa and 12 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
纯度（Purity）
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
数据展示
电泳（SDS-PAGE）
Biotinylated Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Complex Protein on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Biotinylated Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Complex Protein (Cat. No. HLM-H82E5) is more than 95% and the molecular weight of this protein is around 48-60 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Biotinylated Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Complex Protein (Cat. No. HLM-H82E5) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.1-2 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Complex Protein (Cat. No. HLM-H82E5) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-HLA-A2&MAGE-A4 Antibody, Human IgG1 with a linear range of 0.1-1 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Complex Protein (Cat. No. HLM-H82E5) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can Anti-B2M Antibody, Human IgG1 with a linear range of 0.1-2 ng/mL (Routinely tested).
Protocol
活性（Bioactivity）-SPR
Biotinylated Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Complex Protein (Cat. No. HLM-H82E5) captured on Biotin CAP-Series S Sensor Chip can bind Anti-HLA-A2&MAGE-A4 Antibody, Human IgG1 with an affinity constant of 22.3 pM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
用户评价 发表评论
联系我们 
项目合作